Superoxide dismutase activity in nerve cell culture |
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Authors: | R Fried J Ciesielski-Treska M Ledig P Mandel |
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Institution: | (1) Department of Biochemistry, Creighton University Medical School, 68178 Omaha, Nebraska;(2) Centre de Neurochimie du CNRS, 11 Rue Humann, 67085 Strasbourg CEDEX, France |
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Abstract: | Superoxide dismutase (EC 1.15.1.1) activity was assayed in preparations obtained from several clonal lines of nerve cell culture, by enzymatic and nonenzymatic assays. The enzyme was found in dialyzed homogenates of washed cells and in partially purified fractions. The enzyme was also found in cells which had been grown in media containing 5-bromodeoxyuridine, where cell differentiation was observed. |
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