Oxygen requirement in pullulan fermentation |
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| Authors: | Denis Rho Ashok Mulchandani John H T Luong Anh LeDuy |
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| Institution: | (1) Biotechnology Research Institute, National Research Council of Canada, H4P 2R2 Montreal, Quebec, Canada;(2) Department of Chemical Engineering, Laval University, G1K 7P4 Sainte Foy, Quebec, Canada |
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| Abstract: | Summary Oxygen was essential for the biosynthesis of pullulan by Aureobasidium pullulans. In a growth medium, pullulan yield and synthesis rate were proportional to the oxygen availability. However, under controlled oxygen environment in a non-growth medium, the synthesis rate and the yield of pullulan were inversely proportional to the oxygen tension. A relationship between melanin production and oxygen transfer conditions was also observed. The elapsed time prior to the appearance of the pigment was dependent upon the degree of oxygen availability. |
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