The influence of cell cycle kinetics on the radiosensitivity of Down's syndrome lymphocytes |
| |
| Authors: | J C Leonard T Merz |
| |
| Affiliation: | 1. Johns Hopkins Oncology Center, Section of Radiobiology, 600 North Wolfe Street, Baltimore, MD 21205 U.S.A.;1. Medical College of Virginia, Virginia Commonwealth University, Department of Radiology, Division of Radiobiology, 10th and Marshall Streets, Richmond, VA 23298 U.S.A. |
| |
| Abstract: | In agreement with previous work, [60Co]gamma-irradiation shortly after phytohemagglutinin (PHA) stimulation, induces higher frequencies of chromosomal aberrations in trisomy 21 lymphocytes compared to normal controls. However, equal frequencies of chromatid aberrations are induced in fully-stimulated trisomy 21 and normal lymphocytes by irradiation during G2. We have observed that trisomic lymphocytes respond more rapidly to PHA stimulation than normal lymphocytes. Furthermore, we have observed that chromosomal radiosensitivity increases as a function of time after PHA stimulation in normal lymphocytes. When normal lymphocytes are irradiated 8 h after PHA stimulation, the frequencies of chromosomal aberrations induced are comparable to those induced in trisomy 21 lymphocytes irradiated 30 min after PHA stimulation. |
| |
| Keywords: | PHA phytohemagglutinin |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
