| 多焦点多光子显微技术及其研究进展 |
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| 引用本文： | 刘立新.多焦点多光子显微技术及其研究进展[J].激光生物学报,2011,20(6):711-717. |
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| 作者姓名： | 刘立新 |
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| 作者单位： | 西安电子科技大学技术物理学院,陕西西安,710071 |
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| 基金项目： | 中央高校基本科研业务费专项资金，深圳市非共识技术创新项目计划 |
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| 摘 要： | 多焦点多光子显微技术(multifocal multiphoton microscopy,MMM)提高了激发光能的利用率和成像速度,可以实现样品的三维快速多光子激发荧光显微成像,并具有对活体样品损伤小,成像深度大,图像信噪比高等优点.详细阐述了MMM的实现方法及其研究进展,包括同时时间和光谱分辨的MMM(simulta...
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| 关 键 词： | 多焦点多光子显微技术 时间和光谱分辨 变视场扫描 随机扫描 时间相关单光子计数 |
Multifocal Multiphoton Microscopy and Its Research Progress |
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| LIU Lixin.Multifocal Multiphoton Microscopy and Its Research Progress[J].ACTA Laser Biology Sinica,2011,20(6):711-717. |
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| Authors: | LIU Lixin |
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| Institution: | LIU Lixin (School of Technical Physics,Xidian University,Xi’an 710071,Shaanxi,China) |
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| Abstract: | Multifocal multiphoton microscopy（MMM） can greatly improve the utilization of the excitation light and imaging speed,and it can also acquire three-dimensional fluorescence microscopic images by multiphoton excitation with the advantages of high speed,reduced photobleaching and photodamage,enhanced penetration depth and high signal to noise ratio.The realization and research progresses of multifocal multiphoton microscopy are discussed in detail,which include simultaneous time- and spectrum-resolved multifocal multiphoton microscopy（STSR-MMM）,multifocal multiphoton microscopy based on lime-correlated single photon counting（TCSPC-MMM）,multifocal multiphoton microscopy using a field of view zoom scanning protocol and stochastic scanning multifocal multiphoton microscopy（SS-MMM）,etc. |
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| Keywords: | multifocal multiphoton microscopy time- and spectrum-resolved field of view zoom scanning stochastic scanning time-correlated single photon counting |
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