| 紫外光诱导的油菜菌核病菌抗扑海因菌株的特性研究 |
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| 引用本文： | 陈方新,齐永霞,高智谋.紫外光诱导的油菜菌核病菌抗扑海因菌株的特性研究[J].激光生物学报,2011,20(6):729-733. |
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| 作者姓名： | 陈方新 齐永霞 高智谋 |
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| 作者单位： | 安徽农业大学植物保护学院,安徽合肥,230036 |
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| 基金项目： | 公益性行业(农业)科研专项 |
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| 摘 要： | 通过室内紫外光诱导,获得了油菜菌核病菌多株抗扑海因突变体。采用菌丝生长速率法.对诱变得到的抗扑海因菌株的抗药性进行了测定。结果显示,油菜菌核病菌抗扑海因菌株的EC_（50）值分布范围为0.1159-604.2200μg/mL,平均值为117.1363μg/mL,突变株UVIP＇SS-7、UVIP＇SS-8、UVIP＇SS-15、UVIP＇SS-16和UVIP＇SS-18的EC_（50）值均大于160μg/ML。油菜菌核病菌抗扑海因突变株在含扑海因质量浓度为1μg/mL时的菌核产生数量绝大多数都大于不含药PSA平板的菌核数量,而高浓度下菌核几乎不产生。
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| 关 键 词： | 油菜菌核病菌 扑海因 紫外光 抗药性 |
Characteristics of the Resistant Mutants of Sclerotinia sclerotiorum to Iporodione by Ultraviolet Rays |
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| CHEN Fangxin,QI Yongxia,GAO Zhimou.Characteristics of the Resistant Mutants of Sclerotinia sclerotiorum to Iporodione by Ultraviolet Rays[J].ACTA Laser Biology Sinica,2011,20(6):729-733. |
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| Authors: | CHEN Fangxin QI Yongxia GAO Zhimou |
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| Institution: | (College of Plant Protection,Anhui Agricultural University,Hefei 230036,Anhui,China) |
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| Abstract: | In the laboratory,by ultra-violet rays,the iprodione-resistant mutants of Sclerotinia sclerotiorum were induced, and the resistances of iprodione-resistant mutants were tested by rate of mycelia growth.The results showed that the EC_（50） values of iprodione of all iprodione-resistant mutants ranged from 0.1159μg/mL to 604.2200μg/mL,with the average of 117.1363μg/mL.The EC_（50） of UVIP＇SS-7,UVIP＇SS-8,UVIP＇SS-15,UVIP＇SS-16,and UVIP＇SS-18 were higher than 160μg/mL.Sclerotial numbers and weights of the mutants,which cultured in PSA medium with iprodione of 1μg/mL,were more than control with no iprodione.It suggested that in this experiment,the iprodione-resistant strains were easily obtained and there might be resistance risk of iprodione. |
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| Keywords: | Sclerotinia sclerotiorum iprodione ultraviolet rays fungicide-resistance |
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