Genetic mapping of bovine mitochondrial DNA from a single animal |
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| Authors: | WW Hauswirth PJ Laipis ME Gilman TW O&#x;Brien GS Michaels MA Rayfield |
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| Institution: | 1. Department of Medical Microbiology and Immunology, J. Hillis Miller Health Center, University of Florida, Gainesville, FL 32610 U.S.A.;1. Department of Biochemistry and Molecular Biology, J. Hillis Miller Health Center, University of Florida, Gainesville, FL 32610 U.S.A. |
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| Abstract: | Using a physical map of bovine mitochondrial DNA derived from the liver of a single Holstein cow, we have determined the location of the genes specifying the large and small riibosomal RNAs by hybridization analysis and electron microscopic observations of R-loop forms. Also, the position of the origin of DNA replication (D-loop) has been located by electron microscopy. Additionally, the direction of D-loop expansion and the polarity of the large and small ribosomal RNA genes were determined. |
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| Keywords: | Ribosomal RNA D-loop tailing replication origin R-loop mapping DMSO dimethyl sulfoxide SDS sodium dodecyl sulfate |
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