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Isoelectric focusing in polyacrylamide gel carried out with a simple device for power regulation : Application to mammalian growth hormones
Authors:Camillo Secchi  Mariagiulia Cagnasso  Giacomo Resmi  Pier Antonio Biondi
Affiliation:Istituto di Fisiologia Veterinaria e Biochimica, Via Celoria 10, 20133 Milan Italy
Abstract:A unit is described to regulate the electrical power delivered to an isoelectric focusing cell. The device consists in a resistance set, in series with a power supply, which can be adjusted to a suitable resistance value in order to permit only minor changes in wattage. Simultaneous monitoring of voltage and current is also carried out with a pen-recorder adaptor.The improvements in operative conditions achieved by these means are tested by the fractionation of mammalian growth hormones.
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