Isoelectric focusing in polyacrylamide gel carried out with a simple device for power regulation : Application to mammalian growth hormones |
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Authors: | Camillo Secchi Mariagiulia Cagnasso Giacomo Resmi Pier Antonio Biondi |
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Institution: | Istituto di Fisiologia Veterinaria e Biochimica, Via Celoria 10, 20133 Milan Italy |
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Abstract: | A unit is described to regulate the electrical power delivered to an isoelectric focusing cell. The device consists in a resistance set, in series with a power supply, which can be adjusted to a suitable resistance value in order to permit only minor changes in wattage. Simultaneous monitoring of voltage and current is also carried out with a pen-recorder adaptor.The improvements in operative conditions achieved by these means are tested by the fractionation of mammalian growth hormones. |
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