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Use of a serum gradient for separation of polykaryocytes from single cells after fusion with Sendai virus |
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| Authors: | G. Barbanti-Brodano L. Possati G. P. Minelli M. La Placa |
| Affiliation: | Institute of Microbiology, University of Bologna, Bologna, Italy |
| Abstract: | After cell fusion, polykaryocytes and heterokaryocytes were separated from single cells by a calf serum gradient. Fractions enriched with polykaryocytes were thus obtained. Heterokaryocytes with a prevalence of nuclei from either parental cell were distributed in different fractions of the gradient. The efficiency of the separation procedure is evaluated and its possible application to problems in cell hybridization techniques is discussed. |
| Keywords: | Requests for reprints should be addressed to: Dr G. Barbanti-Brodano Istituto di Microbiologia Via San Giacomo 12 I-40126 Bologna Italy. |
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