The Transport and Metabolism of Urea in Chara australis: III. TWO SPECIFIC TRANSPORT SYSTEMS |
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Authors: | WILSON, M. R. O'DONOHUE, S. I. WALKER, N. A. |
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Abstract: | Reciprocal competitive inhibition studies were used to showthat N-methyl-urea (NMU), acetamide and urea all compete forbinding to a common transport system, designated system I andthat this system is one of two specific mechanisms transportingurea in Chara. System I binds urea with a Km of about 03mmol m-3 and is strongly influenced by metabolic controls. SystemI is active and electrogenic and may be energized by the couplingof urea uptake to an influx of protons. This is the first reportof an electrogenic urea transport system in an alga. The secondspecific mechanism for urea transport, designated system II,binds urea with a relatively low affinity (Km c. 70 mmolm-3) and does not transport NMU to a significant extent. SystemII is less subject to metabolic control than system I and, thoughit may be active, is not electrogenic. Key words: Urea, methylurea, proton cotransport, metabolic control |
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