Template Properties of Glucose-Deficient T-Even Bacteriophage DNA

The vegetative DNA isolated from T4-infected Escherichia coli W4597 (UDPG PPase(-)) was about two to six times more active in stimulating protein synthesis in cell-free extracts than that isolated from T4-infected E. coli B06. This suggested that nonglucosylated vegetative DNA may be a better template than the glucosylated form. This view was supported by experiments measuring RNA synthesis on mature T-even DNAs with a range of glucose contents. The extent of (14)C-GTP polymerization was inversely proportional to the glucose content of the DNA. Differences were also observed in both the kind and quantity of polypeptides produced in response to these DNAs.