Molecular cloning and expression ofThiobacillus ferrooxidans chromosomal ribulose bisphosphate carboxylase genes inEscherichia coli |
| |
| Authors: | Dr. Tomonobu Kusano Kazuyuki Sugawara Chihiro Inoue Nobuhiro Suzuki |
| |
| Affiliation: | (1) Research Center, Dowa Mining Co. Ltd., Hachioji, Tokyo, Japan;(2) Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agriculture, Ohgata, 010-04 Akita, Japan |
| |
| Abstract: | The genes coding ford-ribulose-1,5-bisphosphate carboxylase (RuBPCase) from an iron-oxidizing bacterium,Thiobacillus ferrooxidans, were cloned into anEscherichia coli plasmid, pUC18. The recombinant plasmid, termed pTR11, contained a 4.0-kb PstI fragment including the entire coding regions for both large and small subunits of RuBPCase.Escherichia coli carrying pTR11 did not show any CO2-fixing activity. However, a derivative plasmid with an appropriate deletion, which was placed under the control of atac promoter, conferred ribulose bisphosphate-dependent CO2-fixing activity on the host cell. Analysis of gel-filtration chromatography of the RuBPCase synthesized inE. coli revealed that it had a hexadecameric form like the native enzyme ofT. ferrooxidans. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
