The phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis |
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| Authors: | Seigo Shima Miyoko Yanagi Hiroshi Saiki |
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| Institution: | Department of Biotechnology, Central Research Institute of Electric Power Industry, Abiko 1646, Abiko-city, Chiba 270-11, Japan |
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| Abstract: | Abstract Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phylogenetic analyses show strain 3H-1 is closely related to Aquifex pyrophilus and may be located in the deepest branch within the eubacterial phylogenetic tree. Sulfur-dependency of the ancestral eubacterium is also discussed. |
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| Keywords: | 16S rRNA Hydrogenobacter acidophilus Aquifex Phylogeny Eubacterial ancestry |
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