Labelling oligonucleotides to high specific activity (I). |
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| Authors: | A G Craig D Nizetic and H Lehrach |
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| Institution: | Genome Analysis Laboratory, Imperial Cancer Research Fund Laboratories, London, UK. |
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| Abstract: | The normal procedure for labelling oligonucleotides radioactively is the use of polynucleotide kinase and gamma 32P-ATP. However, this has the disadvantage of only introducing one labelled base per molecule of the oligonucleotide. In this paper we describe an approach based on primer/template combinations using conventional fill-in conditions followed by the release of the labelled sequence by digestion with uracil-DNA glycosylase. |
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