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A genetic map of Peromyscus with chromosomal assignment of linkage groups (a Peromyscus genetic map) |
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| Authors: | Jane Kenney-Hunt Adrienne Lewandowski Travis C Glenn Julie L Glenn Olga V Tsyusko Rachel J O’Neill Judy Brown Clifton M Ramsdell Quang Nguyen Tony Phan Kimberly R Shorter Michael J Dewey Gabor Szalai Paul B Vrana Michael R Felder |
| Institution: | 1. Department of Biological Sciences and Peromyscus Genetic Stock Center, University of South Carolina, Columbia, SC, 29208, USA 5. Department of Biology and Environmental Science, Westminster College, Fulton, MO, 65251, USA 2. Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29801, USA 6. Department of Environmental Health Science, University of Georgia, Athens, GA, 30602-7396, USA 7. Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, 40546-0091, USA 3. Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269-2131, USA 9. Department of Allied Health Sciences, University of Connecticut, Storrs, CT, 06269, USA 8. Gene Expression Division, Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA, 94547, USA 4. Department of Biological Chemistry, University of California, Irvine, CA, 92799, USA |
| Abstract: | The rodent genus Peromyscus is the most numerous and species-rich mammalian group in North America. The naturally occurring diversity within this genus allows opportunities to investigate the genetic basis of adaptation, monogamy, behavioral and physiological phenotypes, growth control, genomic imprinting, and disease processes. Increased genomic resources including a high quality genetic map are needed to capitalize on these opportunities. We produced interspecific hybrids between the prairie deer mouse (P. maniculatus bairdii) and the oldfield mouse (P. polionotus) and scored meiotic recombination events in backcross progeny. A genetic map was constructed by genotyping of backcross progeny at 185 gene-based and 155 microsatellite markers representing all autosomes and the X-chromosome. Comparison of the constructed genetic map with the molecular maps of Mus and Rattus and consideration of previous results from interspecific reciprocal whole chromosome painting allowed most linkage groups to be unambiguously assigned to specific Peromyscus chromosomes. Based on genomic comparisons, this Peromyscus genetic map covers ~83 % of the Rattus genome and 79 % of the Mus genome. This map supports previous results that the Peromyscus genome is more similar to Rattus than Mus. For example, coverage of the 20 Rattus autosomes and the X-chromosome is accomplished with only 28 segments of the Peromyscus map, but coverage of the 19 Mus autosomes and the X-chromosome requires 40 chromosomal segments of the Peromyscus map. Furthermore, a single Peromyscus linkage group corresponds to about 91 % of the rat and only 76 % of the mouse X-chromosomes. |
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