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Improvements to polar 2-D electrophoresis for proteomic applications |
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| Authors: | Rita Polati Daniela Cecconi Ryan Dosselli Mattia Zaccarin Renato Millioni |
| Institution: | 1. Department of Biotechnology, Proteomics and Mass Spectrometry Laboratory, University of Verona, Verona, Italy 2. Centre for Integrative Bee Research (CIBER), ARC CoE in Plant Energy Biology, The University of Western Australia, MCS Building M316, Crawley, 6009, Australia 3. Proteomic Centre of Padova University, Padua, Italy 4. Department of Molecular Medicine, University of Padova, Padua, Italy 5. Chair of Metabolism, Department of Medicine, University of Padova, Padua, Italy |
| Abstract: | Recently, we reported a new way of performing 2-DE, called P-dimensional electrophoresis (2-PE). In this approach, the second dimension is achieved in a radial gel which can accommodate up to six 7 cm long IPG strips simultaneously, improving reproducibility and throughput power in respect to 2-DE. Nevertheless, 2-PE was up to now limited to the use of only short strips because of technical difficulties. Here, we describe how to load longer strips (e.g., 18–24 cm) on 2-PE and report some representative images for a qualitative assessment. |
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