A photometric assay for protease digestion of the proteoglycan subunit. |
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| Authors: | A I Sapolsky J F Woessner D S Howell |
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| Affiliation: | 1. Department of Biochemistry, University of Miami School of Medicine, Miami, Florida 33152 USA;2. Department of Medicine, University of Miami School of Medicine, Miami, Florida 33152 USA |
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| Abstract: | A photometric assay has been developed for the quantitative measurement of the digestion of the proteoglycan subunit by proteases. It is based on forming a complex of proteoglycan subunit and its digestion products with cetyl pyridinium chloride. The digestion products are then selectively released with trichloroacetic acid. These products are quantitated by their uronic acid content. The assay can detect digestion of proteoglycan by as little as 50 ng of trypsin. It offers the further advantage of rapid quantitation of activityin a large number of samples. The assay has been successfully applied to the study of neutral protease activity in human articular cartilage. |
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