Transgenic Rice Expressing Ictb and
FBP/Sbpase Derived from Cyanobacteria Exhibits Enhanced
Photosynthesis and Mesophyll Conductance to CO2
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| Authors: | Han Yu Gong Yang Li Gen Fang Dao Heng Hu Wen Bin Jin Zhao Hai Wang Yang Sheng Li |
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| Affiliation: | 1 State Key Laboratory for Hybrid Rice, College of Life Sciences, WuhanUniversity, Wuhan, China, ; 2 Engineering Research Centre for the Protection and Utilization ofBioresource in Ethnic Area of Southern China, South-Central University forNationalities, Wuhan, China, ; Institute of Genetics and Developmental Biology, ChineseAcademy of Sciences, CHINA, |
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| Abstract: | To find a way to promote the rate of carbon flux and further improve thephotosynthetic rate in rice, two CO2-transporting and fixing relevantgenes, Ictb and FBP/Sbpase, which were derivedfrom cyanobacteria with the 35SCaMV promotor in the respective constructs, weretransformed into rice. Three homologous transgenic groups withIctb, FBP/Sbpase and the two genescombined were constructed in parallel, and the functional effects of these twogenes were investigated by physiological, biochemical and leaf anatomy analyses.The results indicated that the mesophyll conductance and net photosynthetic ratewere higher at approximately 10.5–36.8% and 13.5–34.6%,respectively, in the three groups but without any changes in leaf anatomystructure compared with wild type. Other physiological and biochemicalparameters increased with the same trend in the three groups, which showed thatthe effect of FBP/SBPase on improving photosynthetic capacity was better thanthat of ICTB and that there was an additive effect in ICTB+FBP/SBPase. ICTBlocalized in the cytoplasm, whereas FBP/SBPase was successfully transported tothe chloroplast. The two genes might show a synergistic interaction to promotecarbon flow and the assimilation rate as a whole. The multigene transformationengineering and its potential utility for improving the photosynthetic capacityand yield in rice were discussed. |
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