The human carcinoma cell line A431 possesses large numbers of functional beta-adrenergic receptors |
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| Authors: | C Delavier-Klutchko J Hoebeke A D Strosberg |
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| Affiliation: | 1. ARC Research Group on Photosynthesis, Department of Botany, The University, Sheffield S10 2TN, England;2. Department of Environmental Biology, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia |
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| Abstract: | The existence of beta-adrenergic receptors was demonstrated on whole A431 cells as well as A431 membrane preparations by means of binding assays using the hydrophobic 1-[3H]dihydroalprenolol and the hydrophilic antagonist [3H]CGP-12,177 as beta-adrenergic ligands. Binding was stereospecific. The receptors, as shown by competition studies, proved to be of the beta 2-subtype and appeared functional in the stimulation of adenylate cyclase. The number of receptors per cell and the yield of receptor sites/mg membrane protein render the A431 cell a useful tool for the study of human beta-adrenergic receptors. |
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| Keywords: | Reconstitution Photosystem II complex Light-harvesting chlorophyll-protein complex Proteoliposome DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea DCPIP, 2,6-dichlorophenolindophenol DPC, diphenylcarbazide LHCP, light-harvesting chlorophyll-protein PS, photosystem Chlorophyll Chl |
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