On-line monitoring of antifouling and fouling-release surfaces using bioluminescence and fluorescence measurements during laminar flow |
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Authors: | A A Arrage N Vasishtha D Sundberg G Bausch H L Vincent D C White |
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Institution: | (1) Center for Environmental Biotechnology, University of Tennessee, 10515 Research Dr, Ste 300, 37932 Knoxville, TN, USA;(2) Polymer Research Group, Dept of Chemical Engineering, University of New Hampshire, 03824 Durham, NH, USA;(3) Dow Corning Corp, 48686 Midland, MI, USA;(4) HV Enterprises, 48640 Midland, MI, USA;(5) Environmental Sciences Division, Oak Ridge National Laboratory, 37831 Oak Ridge, TN, USA;(6) Present address: Southwest Research Institute, 78228-0510 San Antonio, TX, USA |
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Abstract: | A laminar flow biofilm-monitoring system was used to determine the efficacies of three antifouling (AF) coatings and five fouling-release (FR) coatings againstVibrio harveyi attachment. On-line measurements of tryptophan fluorescence and bioluminescence from each coating, normalized to an upstream stainless steel coupon, were used to determine the effects of AF and FR surfaces on biofilm formation. The AF coatings consisted of 5, 10, and 35 wt% Sea Nine 211 (C9211) incorporated into a vinyl copolymer. Both the 10 and 35 wt% coatings significantly inhibited biofilm biomass development measured by tryptophan fluorescence compared to the stainless steel control.V. harveyi bioluminescence was significantly greater than tryptophan fluorescence in cells attached to these coatings, suggesting that bioluminescence expression may be a marker for cellular stress or toxicity in biofilms. Five different polydimethylsiloxane (PDMS) FR coatings did not inhibit biofilm formation under low flow conditions. However, four PDMS coatings demonstrated decreased biomass levels compared to stainless steel after exposure to a shear stress of 330 dynes cm–2. There was no toxic additive in these coatings; bioluminescence and tryptophan fluorescence were proportional. |
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Keywords: | antifouling biofilms bioluminescence Sea nine 211 fluorescence polydimethylsiloxane Vibrio harveyi on-line monitoring |
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