Dimensional changes in cells and tissues during specimen preparation for the electron microscope. |
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Authors: | M V King |
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Affiliation: | Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509. |
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Abstract: | Studies on dimensional changes incurred during preparation of tissue specimens for the transmission and scanning electron microscopes are reviewed, with emphasis on quantitative measurements pertinent to morphometry and three-dimensional reconstruction. The scope of the review includes fixation, dehydration, plastic embedment, critical-point drying, and freeze-drying. Recommendations are presented for monitoring dimensional changes; a strategy for the choice of method of specimen preparation is outlined. |
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