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Determination of bacterially derived auxins using a microplate method
Authors:M Sarwar  RJ Kremer
Institution:Department of Soil and Atmospheric Sciences and USDA-ARS, Cropping Systems and Water Quality Research Unit, University of Missouri, Columbia, MO, USA
Abstract:Rapid and efficient methods for determining the ability of soil and rhizosphere bacteria to produce key metabolites which are useful in growth promotion or suppression of plant growth are needed. A microplate method was developed and compared to a standard method for assays of auxin compounds produced by bacteria. The microplate method was similar to the standard method in accuracy of determination, required less chemical reagents, and considerably reduced the time required for analyses.
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