Nucleic acid hybridisation with a probe specific for 3 aminoglycoside acetyltransferase type IV: A survey of resistance to apramycin and gentamicin in animal strains of Escherichia coli |
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Authors: | Elisabeth Chaslus-Dancla Guy Gerbaud Jean-Pierre Lafont Jean-Louis Martel Patrice Courvalin |
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Affiliation: | Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly, 37380 Monnaie, France;Unitédes Agents Antibactériens, UA CNRS 271, Institut Pasteur, 75724 Paris, Cedex 15, France;Laboratoire National de Pathologie Bovine, 69342 Lyon, Cedex 02, France |
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Abstract: | Abstract Resistance to apramycin due to production of a 3-aminoglycoside acetyltransferase type IV (AAC(3)IV) has recently been detected among Gram-negative bacteria isolated in France from bovine clinical samples. 24 apramycin-resistant Escherichia coli strains isolated over the country, and epidemiologically unrelated, were studied by colony hybridization using an intragenic probe specific for AAC(3)IV. The results obtained indicated that the structural gene for the acetyltransferase was present in all the isolates tested and in the corresponding apramycin-resistant transconjugants. This observation demonstrates that resistance to apramycin by acetylation of the antibiotic has spread very rapidly in bovine Gram-negative bacteria. |
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Keywords: | Gene probe hybridisation aminoglycoside resistance animal strains |
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