Abstract: | Quantitative differentiation of microbial activity in wheat straw substrate is described after different heat pre-treatments and addition of water during solid-state fermentation. All the 28 tested strains of tropical and sub-tropical edible mushrooms colonized sterile wheat straw. Substrate pre-treated at 25°C was primarily colonized by Coprinus sp. and other competitive microorganisms, and had the highest pH values. With some exceptions, increasing rates of growth occurred with substrate pre-treatment at 60 and 90°C. Best growth and highest speed of colonization were on sterilized straw. Heat pre-treatment of cereal straw at 60 and 90°C should be sufficient for commercial cultivation processes. Thus, a short fermentative pre-treatment could reduce the risk of infection. The strains tested do not differ significantly from those of temperate climates.R. Maziero was with the Instituto de Botânica, CP 4005, 01061-970, São Paulo, SP, Brazil, and is now at DISTAM Section of Industrial Microbiology, University of Milano, Via G. Celoria 2, 20133, Milano, Italy. F. Zadrazil is with the Institut für Bodenbiologie, Bundesforchungsanstalt für Landwirtschaft, Bundesallee 50, D 38116 Braunschweig, Germany. |