Effect of sugar type on the efficiency of plant regeneration from protoplasts isolated from shoot tip-derived meristematic nodular cell clumps of Lilium x formolongi hort. |
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Authors: | Toshinari Godo Kazuhiko Matsui Takao Kida Masahiro Mii |
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Institution: | (1) Basic Research Laboratories, Central Research Laboratories, Ajinomoto Co., Inc., Japan;(2) Laboratories of Plant Cell Technology, Department of Horticulture, Chiba University, Japan;(3) Present address: Botanic Gardens of Toyama, 42 Kamikutsiwada, Fuchu-machi, Nei-gun, 939-27 Toyama, Japan |
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Abstract: | Summary Suspension cultures composed of meristematic nodular cell clumps of Lilium x formolongi hort were established from shoot tips placed on MS medium supplemented with 1 mg/l picloram and 30 g/l sucrose, glucose, fructose or sorbitol. Protoplasts isolated from these cultures were embedded in 1 g/l gellan gumsolidified 1/2MS medium with 1 mg/l picloram and the different kinds of sugars at 0.5 M, and cultured at 25 °C in the dark. The highest plating efficiency (13.7%) was obtained when the protoplasts were isolated from the cell clumps which had been subcultured in MS medium containing glucose and were likewise cultured in MS medium supplemented with 0.5 M glucose. Plants were regenerated from the protoplast-derived calli on 1/2MS medium containing 2.5–10 g/l sucrose or 5–10 g/l glucose. These results suggest that the kinds of sugar and concentration are important parameters affecting protoplast isolation, proliferation and plant regeneration in L. x formolomgi hort.Abbreviations FW
fresh weight
- MS
Murashige and Skoog (1962)
- 1/2MS medium
MS medium containing half strength mineral salts
- 1/2MS-0
1/2MS medium containing no growth regulators
- NAA
1-naphthaleneacetic acid
- p-calli
protoplast-derived calli
- PE
plating efficiency
- picloram
4-amino-3,5,6-trichloro-picolinic acid |
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