In vitro translation of distinct mRNAs coding for the precursors of porcine LH subunits |
| |
Authors: | O Pascal H Lejeune J André |
| |
Affiliation: | U.E.R. Lyon-Nord, INSERM U. 34, Hôpital Debrousse, 29 Rue Soeur Bouvier, 69322 Lyon Cedex 05, France |
| |
Abstract: | Conditions are described to characterize and estimate the precursors of porcine LH alpha and beta subunits and indirectly their specific mRNAs. Poly(A) RNAs extracted from castrated male pig anterior pituitaries were translated in a wheat-germ system in the presence of [35S] cysteine and [35S] methionine. The translation products were precipitated by antisera directed against reduced and carboxymethylated LH alpha and beta subunits and analyzed by high resolution electrophoresis. It is shown that the precursors of pLH alpha and beta subunits are located in two distinct congruent to 15 K proteins and represent--on the basis of the incorporation of the [35S] labeled aminoacids into proteins--congruent to 0.12% and 0.05% respectively of the total translation products. It is suggested that in the pig, as in other species, the LH alpha and beta subunits are encoded by two distinct mRNAs, and at variance with other species the leader sequence of LH alpha mRNA is longer than that of LH beta mRNA. |
| |
Keywords: | LH luteinizing hormone FSH follicle-stimulating hormone poly(A) RNA polyadenylic acid rich RNA SDS sodium dodecyl sulfate TCA trichloracetic acid RCM reduced and carboxymethylated pLH porcine LH bLH bovine LH K kilodaltons hCG human chorionic gonadotropin TSH thyreostimulating hormone |
本文献已被 ScienceDirect PubMed 等数据库收录! |