Universal Primers for Amplification of Mitochondrial Small Subunit Ribosomal RNA-Encoding Gene in Scleractinian Corals |
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Authors: | Chaolun Allen Chen Jr-Kai Yu |
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Institution: | (1) Institute of Zoology, Academia Sinica, Nankang 115, Taipei, Taiwan, TW |
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Abstract: | We describe the construction of polymerase chain reaction primers designed to amplify a portion of the mitochondrial (mt)
small subunit ribosomal (SSU) RNA-encoding genes in scleractinian corals. Combinations of cloning and sequencing show that
the amplified fragments are between 694 and 896 bp in length. Alignment of the amplified DNA sequences to the published mt
SSU rRNA genes of Metridium senile and Sarcophyton glaucum indicates several conserved regions among actiniarian, corallimorpharian, octocorallian, and scleractinians, suggesting this
primer set can successfully amplify over 80% of the mt SSU rDNA region of scleractinian corals. Surveys of sequence variation
and estimation of the rate of evolution show an extremely slow divergence of the SSU rRNA gene in the family Acroporidae.
Received June 11, 1999; accepted October 4, 1999. |
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Keywords: | :Acropora mitochondrial small subunit ribosomal RNA slow divergence |
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