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| 芽胞杆菌抗菌活性和纤维素酶活性的测定 | |
| 引用本文： | 张红英,金钺,李娟,陈丽颖,崔保安.芽胞杆菌抗菌活性和纤维素酶活性的测定[J].中国微生态学杂志,2009,21(9):801-803. |
| 作者姓名： | 张红英 金钺 李娟 陈丽颖 崔保安 |
| 作者单位： | 1. 河南农业大学牧医工程学院,郑州450002;河南省动物性食品安全重点实验室,郑州,450002 2. 河南农业大学牧医工程学院,郑州,450002 3. 河南农业大学牧医工程学院05级生物工程专业,郑州,450002 |
| 基金项目： | 河南省基础与前沿技术研究计划项目 |
| 摘 要： | 目的筛选鸡用益生菌。方法通过牛津杯法、混菌法和DNS分光光度法对从鸡肠道分离的10株芽胞杆菌进行抑菌效果及纤维素酶活性测定。结果编号为Y1、Y5、Y7的3株芽胞杆菌对测试菌具有较好抑菌效果，编号为Y2、Y4、Y5的3株芽胞杆菌具有较高的产酶活性。结论从分离的10株芽胞杆菌中筛选出1株（编号为Y5）兼具较好抑菌效果和较高产酶活性的地衣芽胞杆菌，具有作为饲用益生菌开发的潜能。 |
| 关 键 词： | 芽胞杆菌 抗菌活性 纤维素酶 |
Determination of antibiotic and cellulose enzyme activity of Bacillus |
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| ZHANG Hong-ying,JIN Yue,LI Juan,CHEN Li-ying,CUI Bao-an.Determination of antibiotic and cellulose enzyme activity of Bacillus[J].Chinese Journal of Microecology,2009,21(9):801-803. | |
| Authors: | ZHANG Hong-ying JIN Yue LI Juan CHEN Li-ying CUI Bao-an |
| Institution: | ZHANG Hong-ying , JIN Yue , LI Juan , CHEN Li-ying , CUI Bao-an ( 1. Technological College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University,Zhengzhou 450002, China; 2. Key laboratory of Animal Food Safety, Henan Province, Zhengzhou 450002, China ;3. Grade 05 Bio-engineering in Technological College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China) |
| Abstract: | Objective Screening probiotics for chicken. Method Antibiotic activity were tested by Oxford Cup and mixing culture, while the cellulose enzyme activity of the strains were detected by CMC-Na plate culture and DNS spectrophotometric way. Result 3 bacillus strains ( named as Y1, Y5, Y7) showed good antibiotic activity; 3 bacillus strains (named as Y2, Y4, Y5) possessed higher ability for cellulose enzyme production. Conclusion 1 strain numbered as Y5 was selected for potential probiotics which had higher activity producing cellulose enzyme as well as inhibiting bacteria. |
| Keywords: | Bacillus Antibiotic activity Cellulose enzyme |
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