ITS1 intra-individual variability of Ascaris isolates from Brazil |
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| Authors: | Daniela Leles Adauto Araújo Ana Carolina Paulo Vicente Alena Mayo Iñiguez |
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| Affiliation: | 1. Laboratório de Paleoparasitologia, Escola Nacional de Saúde Pública Sérgio Arouca, Brazil;2. Laboratório de Genética Molecular de Microorganismos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil;1. The George Washington University, Department of Epidemiology and Biostatistics, 2175 K Street, NW Suite 500, Washington, DC 20037, United States;2. National Institutes of Health, University of the Philippines Manila, 623 Pedro Gil St., Taft Avenue, Ermita, Manila 1000, Philippines;3. National Institutes of Health and College of Public Health, University of the Philippines Manila, 623 Pedro Gil St., Taft Avenue, Ermita, Manila 1000, Philippines;1. School of Health & Sport Sciences, University of the Sunshine Coast, Locked Bag 4, Maroochydore, QLD 4558, Australia;2. QIMR Berghofer Medical Research Institute, 300 Herston Road, Herston, QLD, Australia;3. Menzies School of Health Research, Charles Darwin University, Casuarina, NT, Australia;4. National Institutes of Health, Bethesda, MD, United States;5. School of Medicine, University of Queensland, Herston, QLD, Australia;1. Centro de Desenvolvimento Tecnológico/Biotecnologia, Universidade Federal de Pelotas, CP 354, CEP 96010-900, Pelotas, RS, Brasil;2. Faculdade de Medicina/Laboratório de Parasitologia, Universidade Federal de Rio Grande, General Osório, S/N, CEP 96200-190, Rio Grande, RS, Brasil;3. Faculdade de Nutrição, Universidade Federal de Pelotas, CP 354, CEP 96010-900, Pelotas, RS, Brasil;1. Institut de Recherche pour le Développement, UMI233/INSERM U1175, Université de Montpellier, Montpellier, France;2. Infectious Diseases Division, Washington University School of Medicine, St Louis, MO, USA;3. Programme National de Lutte contre l''Onchocercose, Direction de l''Epidémiologie et de la Lutte contre la Maladie, Ministère de la Santé et de la Population, Brazzaville, Republic of the Congo |
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| Abstract: | The zoonotic potential of Ascaris infecting pigs has stimulated studies of molecular epidemiology with internal transcribed spacer 1 (ITS1) as the target. The aim of this study was to determine the value of Ascaris ITS1 as a molecular marker through assessing the intra-individual genetic diversity of Ascaris isolates from two geographical areas of Brazil. DNA was extracted from single isolated eggs, ITS1 PCR was performed, and the PCR products were cloned and sequenced. Clone analysis showed high ITS1 intra-individual variability revealed by 2–4 ITS1 genotypes/haplotypes per sample (egg). Two genotypes, G1 and G6, and 13 new haplotypes were detected and characterized. The most prevalent in humans, G1 and/or the Brazilian G6, were detected in all samples. Except for genotype G1, no relationship was observed between Brazilian ITS1 genotypes/haplotypes and those previously described in China, Bangladesh, Japan, United Kingdom, Australia, and Denmark, with respect to geographic origin or host affiliation. However, an association between the two geographically separated Brazilian ITS1 isolates was observed. The ITS1 intra-individual variability revealed in this study indicated that the use of this genetic region to discriminate human and pig Ascaris genotypes should be reconsidered. |
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