Generation of scFv phages specific to Staphylococcus enterotoxin C1 by panning on related antigens |
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| Authors: | Anna V Lomonosova Alexander G Laman Ksenia K Fursova Anna O Shepelyakovskaya Yury V Vertiev Fedor A Brovko Evgeny V Grishin |
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| Institution: | 1.Pushchino Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry; Moscow, Russia;2.Pushchino Branch of Moscow State University; Moscow, Russia;3.Laboratory Gamaleya Research Institute of Epidemiology and Microbiology; Russian Academy of Medical Sciences; Moscow, Russia;4.Laboratory of Neuroreceptors and Neuroregulators; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry; Russian Academy of Sciences; Moscow, Russia |
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| Abstract: | A method for generation of highly specific miniantibodies within the phage particle has been developed and used to produce antibodies against Staphylococcus enterotoxin type C1. Under successive panning of the non-immune phage miniantibody (scFv) library with enterotoxins SE (types A, B, C1, D, E, G and I) adsorbed on the plate surface, we generated 11 individual phage clones to Staphylococcus enterotoxin type C1. Five of them interacted specifically only with SEC1 and had no cross-reactions with the other enterotoxins.Key words: scFv, phage display, antibody specificity, Staphylococcus aureus enterotoxins |
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