Systematics of the subfamily Haplorchiinae (Trematoda: Heterphyidae), based on nuclear ribosomal DNA genes and ITS2 region |
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| Authors: | Urusa Thaenkham Paron Dekumyoy Chalit Komalamisra Megumi Sato Do Trung Dung Jitra Waikagul |
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| Institution: | 1. Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand;2. Department of Parasitology, Faculty of Veterinary Medicine, Universidade Federal do Tocantins, Araguaina, Tocantins, Brazil;3. National Institutes of Malariology, Parasitology, and Entomology, Hanoi, Vietnam;1. Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA;2. Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA;1. Department of Parasitology, and Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;2. Department of Microbiology, University of Medicine 2, Yangon, Ministry of Health and Sport, Myanmar;1. WHO Collaborating Centre for Research and Control of Opisthorchiasis (Southeast Asian Liver Fluke Disease), Tropical Disease Research Laboratory, Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;2. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand;3. Liver Fluke and Cholangiocarcinoma Research Center, Khon Kaen University, Khon Kaen 40002, Thailand;1. Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand;2. Center of Excellence in Animal, Plant and Parasitic Biotechnology, Srinakharinwirot University, Bangkok 10110, Thailand |
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| Abstract: | Phylogenetic relationships of 6 species in the trematode subfamily Haplorchiinae were analyzed using small and large subunit of ribosomal DNA genes (18S rDNA and 28S rDNA) and internal transcribed spacer subunit II (ITS2) region as molecular markers. Maximum Likelihood and Bayesian inference analyses of combined rDNAs and ITS2 indicated a close relationship between the genera Haplorchis and Procerovum, while these two genera were distinct from Stellantchasmus falcatus. These phylogenetic relationships were consistent with the number of testes but not with the characters of the modification of the seminal vesicle or of the ventral sucker. Although three Haplorchis spp. were, together with Procerovum, in the same cluster, their mutual topology was incongruent between rDNA and ITS2 trees. Phylogenetic analyses using other molecular markers with more species are necessary to work out solid phylogenetic relationships among the species in this subfamily. |
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