Protective immunity against lethal anaphylactic reaction in Toxoplasma gondii-infected mice by DNA vaccination with T. gondii-derived heat shock protein 70 gene |
| |
Authors: | Akitoshi Kikumura Hao Fang Hye-Seong Mun Noriko Uemura Masayuki Makino Yusuke Sayama Kazumi Norose Fumie Aosai |
| |
Affiliation: | 1. Department of Parasitology, Shandong University School of Medicine, Jinan, Shandong Province 250012, People''s Republic of China;2. Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People''s Republic of China;3. Department of Pediatrics, Provincial Hospital Affiliated to Shandong University, Shandong University School of Medicine, 250021, Jinan, Shandong Province, People''s Republic of China;4. Medical School, Quzhou College of Technology, Quzhou, 324000, People''s Republic of China;5. Department of Hematology, Qilu Hospital, Shandong University, 250012, Jinan, People''s Republic of China;6. Department of Ji Nan Children’s Hospital, 250022, Jinan, Shandong Province, People''s Republic of China |
| |
Abstract: | Toxoplasma gondii-derived heat shock protein 70 (T.g.HSP70) was proven to induce lethal anaphylactic reaction in T. gondii-infected mice through platelet-activating factor (PAF)-mediated, but not classical IgE-dependent, pathway via TLR4/MyD88 signal pathway. The effector cells generating PAF and causing T.g.HSP70-induced anaphylactic reaction were CD11b+ and CD11c+ cells, although the reaction was enhanced by marked IFN-γ production by CD11b+, CD11c+, CD4+ and CD8+ splenocytes. In the present study, the effects of T.g.HSP70 gene vaccine targeting peripheral dendritic cells were evaluated against T.g.HSP70-induced anaphylactic reaction in T. gondii-infected mice. C57BL/6 mice receiving T.g.HSP70 gene vaccine showed prolonged survival. Platelets of peripheral blood, which completely disappeared during the T.g.HSP70-induced anaphylactic reaction, were partially restored with the T.g.HSP70 gene vaccination. The T.g.HSP70-induced marked production of PAF and IFN-γ from splenocytes of infected mice during the T.g.HSP70-induced anaphylactic reaction was shown to decrease after the T.g.HSP70 gene vaccination. Thus, T.g.HSP70 gene vaccine induced protective immunity against T.g.HSP70-induced PAF-mediated lethal anaphylactic reaction in T. gondii-infected mice. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|