Hydrogen peroxide-induced apoptosis-like cell death in Entamoeba histolytica |
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| Authors: | Anupama Sardar Ghosh Suman Dutta Sanghamitra Raha |
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| Affiliation: | 1. Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea;2. Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, Japan;1. Laboratory of Biotransformation and Phytochemistry, Department of Chemistry, Center of Exact Sciences, State University of Londrina, PR, Brazil;2. Laboratory of Experimental Protozoology, Department of Pathological Sciences, Center of Biological Sciences, State University of Londrina, PR, Brazil;3. Laboratory of Research on Bioactive Molecules, Department of Chemistry, Center of Exact Sciences, State University of Londrina, PR, Brazil;4. Nucleus of Research in Sciences and Technology, University of Franca, SP, Brazil;5. Department of Food Science and Technology, State University of Londrina, PR, Brazil;6. Department of Pathology Science, Clinical Analysis and Toxicology, Health Sciences Center, State University of Londrina, PR, Brazil;7. Laboratory of X-ray Diffraction, Department of Physics, Center of Exact Sciences, State University of Londrina, PR, Brazil |
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| Abstract: | The microaerophilic intestinal parasitic protozoan Entamoeba histolytica has been previously shown to be highly susceptible to oxidative stress induced by hydrogen peroxide. However the mechanism of cell death was not investigated. Studies presented in this paper demonstrate several morphological features in the parasite when exposed to H2O2 which are identical to metazoan apoptotic phenotype indicating a possible apoptosis-like cell death exhibited by E. histolytica in response to H2O2 treatment. Trophozoite cell shrinkage, DNA fragmentation, phosphatidyl serine externalization and increased endogenous reactive oxygen species level have been observed in the protozoan parasite when exposed to 2.0 mM H2O2 for different time periods. Although the parasite genome is completely devoid of any of the homologues of mammalian caspases it still codes for a huge number of cysteine proteases which may take over the apoptotic function of the caspases. But the present study indicates the existence of a cysteine protease independent programmed cell death in the parasite since E-64 the specific cysteine protease inhibitor could not rescue the cells from H2O2 induced apoptosis-like cell death. |
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