The fourth isoform of the adenine nucleotide translocator inhibits mitochondrial apoptosis in cancer cells |
| |
Authors: | Cindy Gallerne Zahia Touat Zhi Xiong Chen Cécile Martel Eleonore Mayola Ossama Sharaf el dein Nelly Buron Morgane Le Bras Etienne Jacotot Annie Borgne-Sanchez Antoinette Lemoine Christophe Lemaire Shazib Pervaiz Catherine Brenner |
| |
Affiliation: | 1. LGBC, CNRS UMR8159, Université Versailles-SQY, PRES Universud Paris, 45, avenue des Etats-Unis, 78035 Versailles, France;2. NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore; Duke-NUS Graduate Medical School, Singapore; Singapore-MITAlliance, Block MD9, 2 Medical Drive, Singapore 117597 Singapore;3. Theraptosis S.A., R&D Laboratories, Biocitech Technology Park, 93230 Romainville, France;4. INSERM U602, Université de Paris-Sud 11, PRES Universud Paris, Hopital Paul Brousse, 14 Avenue Paul Vaillant Couturier, 94805 Villejuif, France;1. Department of Gerontology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;2. Department of Dermatology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;3. Department of Pathology, Dalian Medical University, Dalian 116044, China;1. Department of Thoracic Oncology, The First Affiliated Hospital of Zhejiang University, Hangzhou, China;2. Department of Respiratory Diseases, Shaoxing Municipal Hospital, Shaoxing, China;1. Newark, CA 94560, USA; Number612@yahoo.com |
| |
Abstract: | The adenine nucleotide translocator (ANT) is a mitochondrial bi-functional protein, which catalyzes the exchange of ADP and ATP between cytosol and mitochondria and participates in many models of mitochondrial apoptosis. The human adenine nucleotide translocator sub-family is composed of four isoforms, namely ANT1–4, encoded by four nuclear genes, whose expression is highly regulated. Previous studies have revealed that ANT1 and 3 induce mitochondrial apoptosis, whereas ANT2 is anti-apoptotic. However, the role of the recently identified isoform ANT4 in the apoptotic pathway has not yet been elucidated. Here, we investigated the effects of stable heterologous expression of the ANT4 on proliferation, mitochondrial respiration and cell death in human cancer cells, using ANT3 as a control of pro-apoptotic isoform. As expected, ANT3 enhanced mitochondria-mediated apoptosis in response to lonidamine, a mitochondriotoxic chemotherapeutic drug, and staurosporine, a protein kinase inhibitor. Our results also indicate that the pro-apoptotic effect of ANT3 was accompanied by decreased rate of cell proliferation, alteration in the mitochondrial network topology, and decreased reactive oxygen species production. Of note, we demonstrate for the first time that ANT4 enhanced cell growth without impacting mitochondrial network or respiration. Moreover, ANT4 differentially regulated the intracellular levels of hydrogen peroxide without affecting superoxide anion levels. Finally, stable ANT4 overexpression protected cancer cells from lonidamine and staurosporine apoptosis in a manner independent of Bcl-2 expression. These data highlight a hitherto undefined cytoprotective activity of ANT4, and provide a novel dichotomy in the human ANT isoform sub-family with ANT1 and 3 isoforms functioning as pro-apoptotic while ANT2 and 4 isoforms render cells resistant to death inducing stimuli. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|