Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province,Thailand |
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| Authors: | Malinee T. Anantaphruti Munehiro Okamoto Tippayarat Yoonuan Surapol Saguankiat Teera Kusolsuk Megumi Sato Marcello O. Sato Yasuhito Sako Jitra Waikagul Akira Ito |
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| Affiliation: | 1. Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Thailand;2. Department of Parasitology, Asahikawa Medical College, Asahikawa 078-8510, Japan;3. Department of Parasitology, School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan;1. Department of Parasitology, Faculty of Medicine? Udayana University, Denpasar, Bali, Indonesia;2. Department of Parasitology, Faculty of Veterinary Medicine? Udayana University, Denpasar, Bali, Indonesia;3. Karangasem District Livestock, Fisheries and Marine Office Services, Bali, Indonesia;4. Directorate of Postgraduate, Sari Mutiara Indonesia University, Medan, North Sumatra, Indonesia;5. Department of Parasitology, Asahikawa Medical University, Asahikawa, Japan;6. Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Japan;7. Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yoshida, Japan;8. Chrono-environment Lab, UMR6249, University of Franche-Comté/CNRS and Institut Universitaire de France, Besançon, France;9. Department of Health Science, Asahikawa Medical University, Asahikawa, Japan;10. Department of Veterinary Integrative Biosciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX, USA;1. Department of Parasitology, Asahikawa Medical University, Asahikawa, Japan;2. Medical Research Centre, Institute of Medical Research and Medicinal Plants Studies (IMPM), Ministry of Scientific Research and Innovation, Yaoundé, Cameroon;3. Université des Montagnes, Bangangté, Cameroon;4. Department of Parasitology and Infectious Diseases, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon;1. Unité d’Immunologie, Institut Pasteur de Madagascar, Antananarivo, Madagascar, Madagascar;2. Unité de Spectrométrie de Masse Structurale et Protéomique, Institut Pasteur, Paris, France;3. FOFIFA-Département de Recherches Zootechniques et Vétérinaires/Faculté de Médecine- Département Vétérinaire, Antananarivo, Madagascar;1. Cysticercosis Unit, Instituto Nacional de Ciencias Neurológicas, Lima, Peru;2. Centre for Global Health—Tumbes and Department of Microbiology, School of Sciences, Universidad Peruana Cayetano Heredia, Lima, Peru;3. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA;4. School of Medicine, Universidad Espíritu Santo—Ecuador, Guayaquil, Ecuador;5. Department of Neurological Sciences, Hospital-Clínica Kennedy, Guayaquil, Ecuador;1. Department of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand;2. Liver Disease Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand;3. Research Center in Back, Neck Other Joint Pain and Human Performance (BNOJPH), Khon Kaen University, Khon Kaen, 40002, Thailand;4. Internal Medicine Research Group, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand |
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| Abstract: | A community-based field survey on taeniasis and cysticercosis was performed in two villages in Thong Pha Phum District, Kanchanaburi Province, central Thailand, where 3 Taenia species, T. solium, T. saginata and T. asiatica, are sympatrically occurring. Four (0.6%) out of 667 stool samples were egg-positive for Taenia sp. by Kato–Katz technique. Three out of those four persons and other three persons who were Taenia egg-negative but having a recent (< 1 year) history of discharging worms in stool were treated with niclosamide. One Taenia egg-positive woman was not treated because of severe ascites. After treatment, three persons expelled long strobilae with scolices and two persons expelled strobilae without scolex. One Taenia egg-positive person did not expel any worms post-treatment. Among 5 persons, four expelled a single worm, whereas one expelled multiple worms, may be 6 worms but not confirmed by detection of scolices. One scolex was armed with hooklets, whereas 2 others did not. Multiplex PCR of 10 expelled proglottids (including 6 estimated worms from one patient) revealed that one sample was T. solium, one T. saginata, and 8 T. asiatica. A total of 159 residents agreed to receive a serological test for cysticercosis. By ELISA using partially purified glycoprotein antigen, 9 cases, 5 and 4 from villages A and B respectively, were found to be sero-positive. The five and an additional sample on the border line from village A were evaluated using confirmative immunoblot using recombinant chimeric antigen. Among the six samples, four including the border line sample were confirmed to be cysticercosis by immunoblotting. One of the 4 persons had neurological symptoms with nodular lesions in the brain by computed tomography. These 4 confirmed or suspected cysticercosis cases were free of T. solium worms, but two of them including confirmed NCC case had a past (> 1 year) history of expelling proglottids in the stool. |
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