Biosynthetic Origin of Gibberellins A(3) and A(7) in Cell-Free Preparations from Seeds of Marah macrocarpus and Malus domestica |
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Authors: | Albone K S Gaskin P Macmillan J Phinney B O Willis C L |
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Institution: | School of Chemistry, University of Bristol, Bristol BS8 1TS, United Kingdom. |
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Abstract: | Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A9 (GA9) and 2,3-dehydroGA9 to GA7; GA9 was also metabolized to GA4 in a branch pathway. The preparation from Marah seeds also metabolized GA5 to GA3 in high yield; GA6 was a minor product and was not metabolized to GA3. Using substrates stereospecifically labeled with deuterium, it was shown that the metabolism of GA5 to GA3 and of 2,3-dehydroGA9 to GA7 occurs with the loss of the 1β-hydrogen. In cultures of Gibberella fujikuroi, mutant B1-41a, 1β,2β-2H2]GA4, was metabolized to 1,2-2H2]GA3 with the loss of the 1α- and 2α-hydrogens. These results provide further evidence that the biosynthetic origin of GA3 and GA7 in higher plants is different from that in the fungus Gibberella fujikuroi. |
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