Development of electron spin resonance radical immunoassay for measurement of airborne orchard grass (<Emphasis Type="Italic">Dactylis glomerata</Emphasis>) pollen antigens |
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Authors: | Yuichi Takahashi Masaaki Aoyama Etsuko Abe Takeshi Aita Shigeto Kawashima Nobuo Ohta Masahiro Sakaguchi |
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Institution: | (1) The Yamagata Prefectural Institute of Public Health, Yamagata 990-0031, Japan;(2) RADIA Project, Yamagata Promotional Organization for Industrial Technology Institute for Life Support Technology, Yamagata 990-2473, Japan;(3) Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan;(4) Department of ENT, School of Medicine, Yamagata University, Yamagata 990-9585, Japan;(5) Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara Kanagawa, 229-8501, Japan |
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Abstract: | We have developed a highly sensitive method for the measurement of airborne orchard grass (Dactylis glomerata: Dac g) pollen antigens using an electron spin resonance (ESR) radical immunoassay. In this immunoassay, the lowest detectable
level of Dac g antigen in a sample is 0.1 arbitrary unit; the amount of Dac g antigen in single pollen grains was found to
be as 1.84 units. Thus, Dac g antigens can be detected in amounts of 1/20th of that contained in the grain. This immunoassay
enables early detection of grass pollen antigens. Such information may be useful for patients with grass pollinosis, especially
for those who show symptoms when only low levels of the pollen antigens are present in air. In this study, minor amounts of
Dac g antigen (cross-reactive antigens) were detected in late March, after which the levels gradually increased. The levels
were detected to be 10 units/m3 until the middle of May and then increased after blooming of orchard grass. High levels were maintained until the middle
of June. Some patients who suffer from grass pollinosis show symptoms in late April and early May, when the airborne Dac g
antigen levels were found to be 5–10 units/m3. |
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Keywords: | Airborne pollen Allergen ESR radical immunoassay Orchard grass Japanese cedar |
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