转基因生防菌308R(pCPP430)对番茄根围菌群的影响

研究目的在于了解转基因生防菌308R(pCPP430)对番茄根围菌群代谢能力和群落结构的影响。实验中使用了两种互为补充的方法,即单一碳源利用测试(SCSU)和ERIC-PCR,对分别以308R(pCPP430)悬液、308R悬液和无菌水蘸根处理的番茄植株根围菌群进行比较。SCSU菌落计数的聚类分析表明,308R(pCPP430)和308R处理的根围菌重复之间相似性好,水处理的相似性差。主成分分析也得到了相同的结果。ERIC-PCR聚类结果表明,10种碳源,其中8种水处理和308R处理聚为一类。实验为生防菌与植物的互作提供一些依据,为根围菌群结构研究提供一些新的思路。

Effects of Transgenic Biocontrol Bacterium 308R(pCPP430) on Tomato Rhizosphere Bacterial Community

The study aimed at investigation the effect of the transgenic biocontrol bacteria 308R(pCPP430) on the tomato rhizosphere bacterial communities. Strain 308R (Pantoea agglomerans), an adnascent bacterium isolated from tomato, was a biocontrol bacterium. The recombinant cosmid pCPP430 contained the hrp gene cluster of Erwinia amylovora was transformed into 308R Strain. Transgenic biocontrol bacterium 308R(pCPP430) produced Harpin protein which induce hypersensitive response and disease resistance in plants and, surprisingly, increase plant growth. Two complementary methods, sole-carbon-source utilization tests (SCSU) and ERIC-PCR, were used to compare the characterization of bacterial communities from three groups of tomato roots dipped in sterilized water, 308R suspended liquid and 308R(pCPP430) suspended liquid. The cluster analysis and principal components analysis of the number of colonies from SCSU indicated consistent differences in the rhizosphere of each group. Cluster analysis of SCSU data showed that there were consistent differences in the rhizosphere of each group. The rhizosphere bacteria communities of root dipped with 308R(pCPP 430) and 308R have a relative good replication, but the differences of corresponding replicates in dipped with distilled water was increased by degrees. Cluster analysis of ERIC-PCR fingerprints revealed that most of results (eight in ten) have overlaps between roots dipped in water and those dipped in 308R liquid. The methods used in this study may prove a useful approach for the comparison of bacterial communities.