Genetic relatedness and genetic diversity of ornamental mei (Prunus mume Sieb. et Zucc.) as analysed by AFLP markers |
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Authors: | Chao-Dong Yang Jun-Wei Zhang Xiao-Lan Yan Man-Zhu Bao |
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Institution: | (1) College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China;(2) China Mei Flower Research Center, Wuhan, 430074, China |
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Abstract: | The genetic diversity and genetic relatedness of mei (Prunus mume; 2n = 16) were studied using amplified fragment length polymorphism (AFLP) markers. Eight EcoRI–PstI AFLP primer combinations were applied to 121 distinct genotypes of mei cultivars and related species. A total of 508 AFLP
product bands were produced, of which 382 were polymorphic. The unweighted pair group method with arithmetic averages analysis
was carried out based on these AFLP markers. From this analysis, “Qugeng Mei,” “Yan Mei,” “Chaodou Mei,” and mei cultivars
were seen to share the same P. mume genetic stem. The AFLP data were able to clearly discriminate P. mume from other species in the genus Prunus, with P. armeniaca aligning as its closest related species. Two major groups and nine subgroups of mei flower were identified, and there was
a strong coincidence of these AFLP-based groupings with the respective morphological characters of the accessions. The genetic
diversity of mei accessions was greatest in the Yunnan Province and decreased toward Eastern China and Japan, so supporting
the hypothesis that the southwest of China represents the genetic diversity center of the species. |
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Keywords: | Amplified fragment length polymorphism Genetic relatedness Genetic diversity Prunus mume Sieb et Zucc |
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