Effects of sample preparation conditions on biomolecular solid-state NMR lineshapes |
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| Authors: | David L. Jakeman Dan J. Mitchell Wendy A. Shuttleworth Jeremy N.S. Evans |
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| Affiliation: | (1) Department of Biochemistry and Biophysics, Washington State University, Pullman, WA, 99163-4660, U.S.A |
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| Abstract: | Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of 13P resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 ± 12 Hz and 125 ± 7 Hz respectively, by a variety of methods involving additives and freezing techniques. |
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| Keywords: | EPSP synthase fast freezing narrowing linewidths 13P NMR solid-state NMR trehalose |
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