Heat-shocks prior to treatment of Vicia faba root-tip meristems with maleic hydrazide or TEM reduce the yield of chromatid aberrations

Heat-shocks (10 and 30 min at 40 degrees C) prior to treatment with MH or TEM significantly reduced the yield of metaphases with chromatid aberrations. No such effect was observed when ethanol was used for aberration induction. The 'heat-shock effect' on aberration induction by MH and TEM is comparable to 'clastogenic adaptation' observed after pretreatment ('conditioning') with low clastogen concentrations prior to 'challenging' with high clastogen concentrations; both require unimpaired protein synthesis.