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Enzymatic production of N-acetyl--glucosamine from chitin. Degradation study of N-acetylchitooligosaccharide and the effect of mixing of crude enzymes |
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| Authors: | Hitoshi Sashiwa Shizu Fujishima Naoko Yamano Norioki Kawasaki Atsuyoshi Nakayama Einosuke Muraki Mongkol Sukwattanasinitt Rath Pichyangkura andSei-ichi Aiba |
| Affiliation: | a The Special Division for Human Life Technology, National Institute of Advanced Industrial Science and Technology, 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan;b Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;c Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand |
| Abstract: | N-Acetyl--glucosamine (GlcNAc) was produced from chitin by use of crude enzyme preparations. The efficient production of GlcNAc by cellulases derived from Trichoderma viride (T) and Acremonium cellulolyticus (A) was observed by HPLC analysis compared to lipase, hemicellulase, and pectinase. β-Chitin showed higher degradability than α-chitin when using cellulase T. The optimum pH of cellulase T was 4.0 on the hydrolysis of β-chitin. The yield of GlcNAc was enhanced by mixing of cellulase T and A. |
| Keywords: | N-Acetyl--glucosamine Chitin Crude enzyme |
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