Vibrio vulnificus may produce a metalloprotease causing an edematous skin lesion in vivo |
| |
Authors: | Shin-ichi Miyoshi Yoshihiro Hirata Ken-ichi Tomochika Sumio Shinoda |
| |
Institution: | School of Biological and Health Sciences, University of Westminster, London W1M 8JS, UK |
| |
Abstract: | Abstract Oxalate decarboxylase was detected both intra- and extracellularly in liquid cultures of Coriolus versicolor . Induction of the enzyme by addition of oxalic acid to the medium on day 6 of growth resulted in a 50-fold increase in specific activity in the mycelia and a 30-fold increase in the extracellular specific activity in the media. The protein was isolated and purified from mycelia, and characterised by polyacrylamide gel electrophoresis and Western blotting against a polyclonal antibody raised to oxalate decarboxylase from Collybia velutipes (Basidiomycete). A major protein band of M r 59000 cross-reacted with the antibody. Immunogold-cytochemical labelling of ultra-thin sections of beechwood infected with C. versicolor showed that the enzyme was localised close to the plasma membrane and in intracellular vesicles. |
| |
Keywords: | Oxalate metabolism Basidiomycetes Oxalate decarboxylase Coriolus versicolor |
|