Immunological studies on phosphoenolpyruvate carboxylase in Sedum species with crassulacean acid metabolism or C3 photosynthesis |
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| Authors: | D. MULLER,M. KLUGE,U. GRÖ SCHEL-STEWART |
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| Affiliation: | Institut für Botanik and Institut für Zoologie, Fachbereich Biologic der Technischen Hochschule Darmstadt, German Federal Republic |
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| Abstract: | Abstract From Sedum morganianum, which is a plant species known to have constitutive crassulacean acid metabolism (CAM), phosphoenolpyruvate (PEP) carboxylase (E.C.4.1.1.31) has been extracted and purified by (NH4)2SC4 precipitation, ion exchange chromatography and gel electrophoresis. A specific antibody to this purified enzyme was obtained by immunization of a rabbit. This antibody was used to compare the antigen–antibody reaction of PEP-carboxylases prepared from other Sedum species including constitutive, facultative and non-CAM plants. The experiments revealed partial immunological indentity of PEP-carboxylases obtained from the different sources. |
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