Appearance of sensory nerve terminals in cat muscle spindles stained for NADH-tetrazolium reductase |
| |
Authors: | J Kucera |
| |
Institution: | (1) Department of Neurology, School of Medicine, Boston University, 80 East Concord Street, 02118 Boston, MA, USA |
| |
Abstract: | Summary Cat muscle spindles were studied histochemically in serial transverse sections of the tenuissimus muscle stained for myofibrillar ATPase, cholinesterase or NADH-tetrazolium reductase. The terminal sites of the primary and secondary sensory axons on intrafusal muscle fibers could be demonstrated due to their high NADH-TR activity. This sensory NADH-TR reactivity at the equator and in the juxtaequatorial regions disappeared following spindle chronic de-afferentation, but not after de-efferentation. Spindle poles that carried both primary and secondary sensory endings had a longer periaxial fluid space than poles with primary endings only, and their motor innervation, as determined by staining for ChE, was positioned at a greater distance from the equator. Some of the secondary endings occurred in intrafusal regions that displayed surface fiber ChE activity. The histochemical reaction for NADH-TR represents a simple, rapid and reliable method for studies of the distribution of sensory nerve terminals in the spindle. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|