Characterization of polysaccharides from Hypnea spinella (Gigartinales) and Halopithys incurva (Ceramiales) and their effect on RAW 264.7 macrophage activity |
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Authors: | Roberto T Abdala Díaz Mariana Chabrillón Alejandro Cabello-Pasini Juan Luis Gómez-Pinchetti and Félix L Figueroa |
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Institution: | (1) Department of Ecology. Faculty of Sciences, University of M?laga, Campus de Teatinos, 29071 M?laga, Spain;(2) Mediterranean Photobiology Center, c/Prosper Merime? 7, 29007 M?laga, Spain;(3) University of Baja California, Ensenada, Baja California, Mexico;(4) Centro de Biotecnolog?a Marina, Universidad de Las Palmas de Gran Canaria, Muelle de Taliarte s/n, 35214 Telde, Las Palmas, Spain; |
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Abstract: | Red algae have been reported to be an important source of polysaccharides with potential immunomodulatory properties. The
objective of this study was to characterize the polysaccharides from Halopithys incurva and Hypnea spinella and to evaluate their effect on the synthesis of cytokines by murine cell line RAW 264.7 macrophages. Polysaccharides were
obtained by N-cetylpyridinium bromide precipitation and characterized by Fourier transform-infrared spectroscopy. Their effect
on the activity of RAW 264.7 macrophages was examined by quantification of tumor necrosis factor (TNF)-α, interleukin (IL)-6,
and nitric oxide (NO) production using enzyme-linked immunosorbent assays. The activation of the cytokine IL-6 and NO increased
linearly as the concentration of polysaccharides from H. incurva and Hy. spinella increased. In general, the activation of IL-6 and NO was tenfold greater when macrophages were exposed to polysaccharides
from H. incurva than when exposed to polysaccharides from Hy. spinella. In contrast, TNF-α concentration did not increase when macrophages were exposed to increasing polysaccharide levels. These
results indicate that polysaccharides are strong cytokine IL-6 inducers. |
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