Efficient accumulation of oleic acid in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> caused by expression of rat elongase 2 gene (<Emphasis Type="Italic">rELO2</Emphasis>) and its contribution to tolerance to alcohols |
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Authors: | Hisashi Yazawa Yasushi Kamisaka Kazuyoshi Kimura Masakazu Yamaoka Hiroshi Uemura |
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Institution: | (1) Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 6, Higashi 1-1-1, Tsukuba Ibaraki, 305-8566, Japan; |
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Abstract: | When the cells of Saccharomyces cerevisiae are exposed to high concentration of ethanol, the content of oleic acid (C18:1n-9) increased as the initial concentration
of ethanol increased. Based on this observation, we attempted to confer ethanol tolerance to S. cerevisiae by manipulating fatty acid composition of the cells. Rather than altering OLE1 expression the desaturase making both C16:1n-7 (palmitoleic acid) and C18:1n-9], we introduced elongase genes. Introduction
of rat elongase 1 gene (rELO1) into S. cerevisiae gave cis-vaccenic acid (cis-C18:1n-7) by conversion from C16:1n-7, and the increase in this C18:1 fatty acid did not confer ethanol tolerance to the
cells. On the other hand, the introduction of rat elongase 2 gene (rELO2), which elongates C16:0 to C18:0, drastically increased C18:1n-9 content, and the cells acquired ethanol tolerance, emphasizing
the specific role of C18:1n-9. Furthermore, the transformant of rELO2 also conferred tolerance to n-butanol, n-propanol, and 2-propanol. |
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