Coupling of growth arrest and expression of early markers during adipose conversion of preadipocyte cell lines |
| |
| Authors: | E Z Amri C Dani A Doglio P Grimaldi G Ailhaud |
| |
| Institution: | 1. Departments of Nutrition, Biochemistry and Molecular Medicine, University of Montreal, and Montreal Diabetes Research Center, Centre de Recherche du Centre Hospitalier de l''Université de Montréal (CRCHUM), Montréal, QC, Canada;2. Department of Medicine, McGill University, Montréal, QC, Canada;3. Dasman Diabetes Institute, Dasman 15462, Kuwait;1. Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China;2. Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Central South University, Changsha 410013, China;3. Sanjiu Medical & Pharmaceutical Co., Ltd, Shenzhen 518029, China |
| |
| Abstract: | After growth arrest at the entry of the S phase of the cell cycle, Ob1771 and 3T3-F442A cells, but not 3T3-C2 cells, accumulate lipoprotein lipase and pOb24 mRNA that are early markers of adipose conversion. Removal of the single- or double-thymidine block when cultured cells are present at low density leads first to DNA synthesis and growth resumption, then to a continuous proliferation and a rapid disappearance of these markers. By contrast, growth-arrested Ob1771 cells reinoculated at high density undergo a single round of cell division, maintain high levels of early marker(s) and acquire with time both glycerol-3-phosphate dehydrogenase and lipids. Thus, depending upon the conditions in culture, growth-arrested cells can undergo either a dedifferentiation leading to a loss of early markers or a terminal differentiation leading to the acquisition of late markers. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
