Actin isoforms of insect muscles: Two-dimensional electrophoresis studies |
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| Affiliation: | 1. Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, PR China;2. The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, PR China;3. Department of Translational Medicine, Xiamen Institute of Rare Earth Materials, Chinese Academy of Sciences, Xiamen 361024, PR China;4. College of Engineering and Computer Science, The Australian National University, Canberra 2601, Australia;5. Key Laboratory of Biomedical Engineering of Fujian Province & Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, Xiamen University, Xiamen 361005, PR China;1. Department of Pharmacology, Medical School of Southeast University, Nanjing, China;2. Department of Pathology, Medical School of Southeast University, Nanjing, China;3. Department of Geriatric Neurology, Nanjing Brain Hospital Affiliated with Nanjing Medical University, Nanjing, China;4. Transmission Electron Microscopy Center, Medical School of Southeast University, Nanjing, China;5. Department of Genetics and Developmental Biology, Medical School of Southeast University, Nanjing, China;1. State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization, Kunming University of Science and Technology, Kunming, Yunnan, 650093, China;2. Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, Yunnan, 650093, China;1. Department of Orthopedics, Monmouth Medical Center, 300 2nd Avenue, Long Branch, NJ 07740, USA;2. Rowan University School of Osteopathic Medicine, 1 Medical Center Drive, Stratford, NJ 08084, USA;3. Pediatric Orthopedics, Seaview Orthopedics and Medical Associates, 1200 Eagle Avenue, Ocean, NJ 07712, USA |
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| Abstract: | 1. Two-dimensional electrophoresis was carried out to determine the isoelectric points of actins from thoracic and leg muscles of various kinds of insects (Coleoptera, Orthoptera, Odonata, Hemiptera, Lepidoptera, Hymenoptera and Diptera).2. The isoelectric points of insect muscle actin ranged from 5.6 to 5.9, appreciably more basic than rabbit skeletal muscle actin (α-actin).3. There was usually one predominant isoform of insect muscle actin except that two isoforms were detected in fly and wasp thoracic muscles.4. In a cicada, the isoelectric points of actins from thoracic, leg and sound organ were 5.65, 5.68 and 5.57, respectively.5. Actins from spider thoracic muscle, and crab claw and crayfish claw muscles also showed the isoelectric points of 5.5–5.7.6. Thus arthropod muscle actins appear to have more basic isoelectric points than vertebrate skeletal muscle ones. |
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