Expression of the Arthrobacter viscosus penicillin G acylase gene in Escherichia coli and Bacillus subtilis |
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| Authors: | H Ohashi Y Katsuta M Nagashima T Kamei M Yano |
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| Affiliation: | Central Research Laboratories, Banyu Pharmaceutical Co., Ltd., Tokyo, Japan. |
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| Abstract: | The penicillin G acylase gene cloned from Arthrobacter viscosus 8895GU was subcloned into vectors, and the recombinant plasmids were transferred into Escherichia coli or Bacillus subtilis. Both E. coli and B. subtilis transformants expressed the A. viscosus penicillin G acylase. The enzyme activity was found in the intracellular portion of the E. coli transformants or in the cultured medium of the B. subtilis transformants. Penicillin G acylase production in the B. subtilis transformants was 7.2 times higher than that in the parent A. viscosus. The A. viscosus penicillin G acylase was induced by phenylacetic acid in A. viscosus, whereas the enzyme was produced constitutively in both the E. coli and B. subtilis transformants carrying the A. viscosus penicillin G acylase gene. |
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